Screening of efficient rhizobacteria associated with cauliflower (Brassica oleraceavar. botrytis L.) for plant growth promoting traits
Article Main
Abstract
In the current study, a total of 25 isolates were isolated from the rhizosphere and roots of cauliflower (Brassica oleraceavar. botrytis L.) from the vicinity of Una district of Himachal Pradesh. The isolates were tested in vitro for their ability to solubilise phosphorous and produce siderophore, indole acetic acid (IAA), hydrogen cyanide (HCN) and antifungal metabolites against the soil borne pathogens. Results revealed that out of 25, only 4 rhizospheric isolates (SB5, SB11, SB8 and SB10) have maximum plant growth promoting attributes. The isolates were identified as Bacillus sp. on the basis of Bergey’s manual of systematic bacteriology. The isolate SB11 recorded highest phosphate solubilizing efficiency in solid medium (109.09%) and in liquid medium (350?g/ml). Maximum production of IAA (51.96?g/ml), siderophore (91.41%) and HCN were also observed for the same isolate. Further-more, the isolate SB11 produced highest antifungal metabolite production against Rhizoctoniasolani(37.11%), Sclerotiniasclerotiorum(41.11%), and Pythium sp. (71.11%) causing root rot, stalk rot and damping off diseases in cauliflower, respectively. The selected isolate (SB11) showed optimum growth at a pH of 7.0, 35°C temperature and 2% NaCl. On the basis of multifarious PGP-traits the SB11 isolate has tremendous potential to be used as a bioferti-lizer/bioprotectant for growth promotion and natural protection of cauliflower under low hill conditions of Himachal Pradesh.
Article Details
Article Details
Biofertilizer, Cauliflower, HCN, P-solubilisation, Siderophore
Bakker, A.W. and Schippers, B. (1987). Microbial cyanide production in the rhizosphere to potato yield reduction and Pseudomonas sp. mediated plant growth stimulation. Soil Biol. Biochem., 19 : 451-457
Barea, J.M. (2015). Future challenges and perspectives for applying microbial biotechnology in sustainable agriculture based on a better understanding of plant-microbiome interactions. J. Soil Sci. Plant Nutr., 15 (2):261-282
Bharucha, U.D., Patel, K.C. and Trivedi, U.B. (2013). Antifungal activity of catecholate type siderphore production produced by Bacillus sp. Int. J. Res. Pharma. Sci., 4 (4):528-531
Chauhan, A., Balgir, P.P. and Shirkot, C.K. (2014). Characterization of Aneurinibacillus aneurinilyticus strain CKMV1 as a plant growth promoting rhizobacteria. IJAEB., 7 (1):37-45
Claus, D. and Berkeley, R.C.W. (1986). Genus Bacillus Cohn. 1872. In: Bergey’s manual of systematic bacteriology. Vol 2, Sneath P H A, Mair N S, Sharpe M E and Holt J G (eds.). Baltimore M D: Williams and Wilkins, USA, pp 1105-1139
Chen, Y., Gao, X., Chen, Y., Qin, H., Huang, L. and Han, Q. (2014). Inhibitory efficacy of endophytic Bacillus subtilis EDR4 against Sclerotinia sclerotiorum on rapeseed. Biol. Control., 78 : 67-76
Dinic, Z., Ugrinovic, M., Bosnic, P., Mijatovic, M., Zdravkovic, J., Miladinovic, M. and Josic, M. (2014). Solubilization of inorganic phosphate by endophytic Pseudomonas sp. from french bean nodules. Ratar. Povrt., 51 (2) : 100-105
Edi-Premono, M., Moawad, M.A. and Vleck, P.L.G. (1996). Effect of phosphate solubilizing Pseudomonas putida on the growth of maize and its survival in the rhizosphere. Indonesian J. Crop Sci., 11:13-23
Garcia-Fraile, P., Menendez, E. and Rivas, R. (2015). Role of bacterial biofertilizers in agriculture and forestry. AIMS Bioeng., 2 (3):183-205.
Gholami, A., Shahsavani, S. and Nezarat, S. (2009). The effect of plant growth promoting rhizobacteria (PGPR) on germination, seedling growth and yield of maize. World Acad. Sci. Eng. Technol., 49 : 19-24
Glick, B. R. (2012). Plant growth promoting bacteria: mechanisms and applications. Scientifica. P. 15.doi: 464 10.6064/2012/963401 [www.hindawi.com/journals/scientifica/2012/963401/]
Gupta, A., Gopal, M. and Tilak, K.V. (2000). Mechanism of plant growth promotion by Rhizobacteria. Indian J. Exp. Biol. 38 : 856-862
Gupta, S., Kaushal, R., Kaundal, K., Chauhan, A. and Spehia, R.S. (2015). Efficacy of indigenous plant growth promoting rhizobacteria on capsicum yield and soil health. Res. on Crops, 16 (1):123-132
Jensen, E. S. (1987). Inoculation of pea by application of Rhizobium in planting furrow. Plant Soil, 97 : 63-70
Junaid, J.M., Dar, N.A., Bhat, T.A., Bhat, A.H. and Bhat, M.A. (2013). Commercial biocontrol agents and their mechanism of action in the management of plant pathogens. Int. J. Modern Plant & Anim. Sci., 1 (2) : 39-57
Kaushal, M., Kaushal, R., Thakur, B.S. and Spehia, R.S. (2011). Effect of plant growth-promoting rhizobacteria at varying levels of N and P fertilizers on growth and yield of cauliflower in mid hills of Himachal Pradesh. J. Farm Sci., 1 (1) : 19-26
Kaushal, M. and Kaushal, R. (2013). Screening and characterization of rhizobacterial strains of Bacillus sp. isolated from rhizosphere of cauliflower (Brassica oleracea var. botrytis L.). Afr. J. Microbiol. Res., 7(17):1657-1663
Mandyal, P., Kaushal, R., Sharma, K. and Kaushal, M. (2012). Evaluation of native PGPR isolates in bell pepper for enhanced growth, yield and fruit quality. Int. J. Farm Sci., 2 (2) : 28-35
Mehta, P., Walia, A., Kakkar, N. and Shirkot, C.K. (2014). Tricalcium phosphate solubilisation by new endophyte Bacillus methylotrophicus CKAM isolated from apple root endosphere and its plant growth-promoting activities. Acta Physiol. Plant., 36 (8) : 2033-2045
Mehta, P., Walia, A., Kulshrestha, S., Chauhan, A. and Shirkot, C.K. (2015). Efficiency of plant growth-promoting P-solubilizing Bacillus circulans CB7 for enhancement of tomato growth under net house conditions. J. Basic Microbiol., 55:33-44
Mohanapriya, J., Jagathy, K. and Vanmathiselvi, K. (2016). Alkaline protease production from B. subtilis SBS 402 isolated from sea food processing effluent. Int. J. Adv. Multidiscip. Res., 3(3):20-30
Pikovskaya, R.I. (1948). Mobilization of phosphorus in soil in connection with the vital activity of some microbial species. Mikrobiologiya, 7:362-370
Saharan, B.S. and Nehra, V. (2011). Plant growth promoting rhizobacteria: a critical review. Life Sci. Med. Res., 21 : 36-48
Sahasrabudhe, M.M. (2011). Screening of rhizobia for indole acetic acid production. Ann. Biol. Res., 2 (4) : 460-468.
Schwyn, B. and Neilands, J.B. (1987). Universal chemical assay for the detection and determination of siderophore. Anal. Biochem., 160 : 47-56
Sharma, R., Walia, A., Chauhan, A. and Shirkot C.K. (2015). Multi-trait plant growth promoting bacteria from tomato rhizosphere and evaluation of their potential as bioinoculants. Appl. Biol. Res., 17 : 1-12
Subba Rao, N.S. (1999). Soil microorganism and plant growth. Oxford & IBH publishing Co, New Delhi. pp. 252
Sundara Rao, W.V.B. and Sinha, M.K. (1963). Phosphate dissolving microorganisms in the soil and rhizosphere. Indian J. Agric. Sci., 33 : 272-278
Vejan, P., Abdullah, R., Khadiran, T., Ismail, S. and Boyce, A.N. (2016). Role of plant growth promoting rhizobacteria in agricultural sustainability: a review. Molecules, 21 : 573
Vincent, J.M. (1947). Distortion of fungal hyphae in the presence of certain inhibitors. Nature, 150 : 158-850.
Wani, P.A., Khan, M.S. and Zaidi, A. (2008). Chromium-reducing and plant growth-promoting Mesorhizobium improves chickpea growth in chromium-amended soil. Biotechnol. Lett., 30 : 159-163
This work is licensed under Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) © Author (s)
