The study was undertaken to determine the prevalence and treatment of antibiotic resistant mastitis in dairy cows. The predominant resistant causative pathogen was Escherichia coli (50.64 %) followed by S. aureus (44.25 %) and Methicillin resistant Staphylococcal aureus (5.11%).These isolates were found sensitive to gentamicin, enrofloxcain, amoxicillin+sulbactam, ceftriaxone and resistant to amoxicillin, oxytetracycline, penicillin G and oxacillin. In all the treatment groups of E. coli, S. aureus and MRSA mastitis, the post treatment pH, SCC was significantly (P < 0.01) decreased when compared to pre treatment pH, SCC values and the post treatment electrical conductivity was significantly (P < 0.01) increased when compared to pre treatment electrical conductivity value. In E. coli mastitis, treated with amoxicillin+sulbactam, ceftriaxone, enrofloxacin and gentamicin showed 74.1%, 67.75 %, 76.67 % and 64.52 % clinical recovery and in S. aureus mastitis, showed 65.25 %, 65.25 %, 72.43 % and 68.98 % clinical recovery. In MRSA mastitis, enrofloxacin was found to be highly effective in comparison to amoxicillin+sulbcactam.
Antimicrobial sensitivity, Bovine mastitis, E. coli, Electrical conductivity, S. aureus
CLSI, Clinical and Laboratory Standard Institute. (2008). In: Approved standard M31-A3(3rd edn). Clinical and Laboratory Standards Institute, Wayne, PA. pp. 65-72.
Edward, M., Anna, K., Michal, K., Henryka, L. and Krystyna, K. (2002). Antimicrobial susceptibility of Staphylococci isolated from mastitic cows. Bulletin of Veterinary Institute in Pulawy, 289-294.
Evira publications. (2009). Recommendations for the use of antimicrobials against the most common infectious diseases in animal. Finnish Food Safety Authority Evira.www.evira.fi/uploads/WebShopFiles/1242284452057.pdf
Hoeben, B.D., Monfardini, E., Burvenich, C. and Hamann, J. (2000). Treatment of acute Escherichia coli mastitis in cows with enrofloxacin: effect on clinical signs and chemiluminescence of circulating neutrophils. Journal of Dairy Research, 67(4): 485-502.
Hui, L.I., Wu, G., Li, J., Tangi, S., Xiao, X., Xue, Y., Dingi, S., Zhangi, S. and Sheni, J. (2013). Pharmacokinetics and pharmacodynamics of a novel amoxicillin / sulbactam / prednisolone intramammary infusion in lactating cows after repeated administrations. Journal of Veterinary Medical Science,75(12):1601-7.
Karthikeyan, A. (2003). Clinicopathological and ultrasonographic studies in bovine mastitis. M.V.Sc., Thesis submitted to Tamil Nadu Veterinary and Animal Sciences University, Chennai, India.
Kenar, B., Kuyucuoglu, Y. and Seker, E. (2012). Antibiotic susceptibility of coagulase-negative staphylococci isolated from bovine subclinical mastitis in Turkey. Pakistan Veterinary Journal, 32: 390-393.
Kumar, R.., Yadav, B.R. and Singh, R.S. (2010). Genetic determinants of antibiotic resistance in Staphylococcus aureus isolates from milk of mastitic crossbred cattle. Current Microbiology, 60: 379â€“386.
Kumar, R., Yadav, B.R. and Singh, R.S. (2011). Antibiotic resistance and pathogenicity factors in Staphylococcus aureus isolated from mastitic Sahiwal cattle. Journal of Biological Sciences, 36: 175-188.
Lee, J.H. (2003). Methicillin(oxacillin)-resistant Staphylococcus aureus strains isolated from major food animals and their potential transmission to humans. Applied Environmental Microbiology, 69: 6489â€“6494.
Loeffler, A. and Lloyd, D.H. (2010). Companion animals: a reservoir for methicillin-resistant Staphylococcus aureus in the community?. Epidemiology of Infection, 138(5): 595-605.
Martineau, F., Picard, F.J., Lansac, N., MÃ©nard, C., Roy, P.H., Ouellette, M. and Bergeron, M.G. (2000). Correlation between the resistance genotype determined by multiplex PCR assays and the antibiotic susceptibility patterns of Staphylococcus aureus and Staphylococcus epidermidis. Antimicrobial Agents Chemotheraphy, 44: 231-238.
Olsen, J.E., Christensen, H. and Aarestrup, F.M. (2006). Diversity and evolution of blaZ from Staphylococcus aureus and Coagulase negative Staphylococci. Journal of Antimicrobial Chemotherapy, 57: 450-460.
Piddock, L.J.V. (1996). Does the use of antimicrobial agents in veterinary medicine and animal husbandry select antibiotic resistant bacteria that infect man and compromise antimicrobial chemotherapy? Journal of Antimicrobial Chemotherapy, 38: 1-3.
Prescott, J.F. and Baggot, J.D. (1994). Antimicrobial therapy in veterinary medicine. 2nd Ed. Academic Press New York.
Riffon, R., Sayasith, K., Khalil, H., Dubreuil, P., Drolet, M. and Lagace, J. (2001). Development of a rapid and sensitive test for identification of major pathogens in bovine mastitis by PCR. Journal of Clinical Microbiology, 39(7): 2584-2589.
Sumathi, B.R., Veeregowda, B.M. and Amitha, R.G. (2008). Prevalence and antibiogram profile of bacterial isolates from clinical bovine mastitis. Veterinary World, 1(8): 237-238.
Tufani, N.A., Makhdoomi, D.M. and Hafiz, A. (2012). Epidemiology and therapeutic management of bovine mastitis. Indian Journal of Animal Research, 46(2): 148 - 151.
Turutoglu, H., Ercelik, S. and Ozturk, D. (2006). Antibiotic resistance of Staphylococcus aureus and Coagulase-negative Staphylococci isolated from bovine mastitis. Bulletin of Veterinary Institute in Pulawy, 50: 41-45.
Yugueros, J., Temprano, A., Sanchez, M., Luengo, J. and Naharro, G. (2001). Identification of Staphylococcus spp. by PCR-restriction fragment length polymorphism of gap gene. Journal of Clinical Microbiology, 39:3693â€“3695.
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