A change in enzyme activity in seeds due to ageing is a topic of scientific importance. Vigour is essentially a physiological phenomenon influenced by the reserved metabolites, enzyme activities and growth regulators. The exact cause of loss of seed vigour and viability is still unknown as deterioration of seed is a complex process. In the presence of oxygen, ageing of seed can lead to peroxidative changes in polyunsaturated fatty acids. The free radical -induced non-enzymatic peroxidation, which has the potential to damage membrane, is likely to be a primary cause of deterioration of stored seeds. Certain anabolic enzymes help in maintaining viability while some catabolic enzymes decrease viability. The seed catalase and peroxidase activity seem to be decreased during storage. The results revealed that the peroxidase enzyme activity decreased from 0.236 to 0.444 OD 10 min-1 when storage period increased. A decrease in catalase activity from 0.454 to 0.444 Î¼g H2O2 mg-1 min-1 followed by a small increase from 0.434 to 0.452 Î¼g H2O2 mg-1 min-1 was observed during storage. But the activity of lipase enzyme increased from 0.236 to 0.231 meq min-1g-1 of sample when the storage period was increased. The study would help to know the deterioration pattern of stored groundnut seeds.
Enzyme changes, Groundnut, Seed, Storage
Bhanuprakash, K., Yogeesha, H.S. and Arun, M.N. (2010). Physiological and biochemical changes in relation to seed quality in ageing bell pepper seed. Indian Journal of Agricultural Sciences, 80(9): 777â€“780.
Cakmak, T., AtÄ±cÄ±, O. and Agar, G. (2009). The natural aging-related biochemical changes in the seeds of two legume varieties stored for 40 years. Acta Agric. Scand. Sect. B - Plant Soil Sci. 60 (4): 353-360.
Demirkaya, M., Dietz, K.J. and Sivritepe, H.O. (2010). Change in antioxidant enzymes during ageing of onion seed. Notulai Botanicae Horti Agrobotanici Cluj-Napoca, 38: 49â€“50.
Gidrol, X., Serghini, H. Noubhani, A. Mocoquot, B. and Mazliak, P. (1989). Biochemical changes induced by accelerated aging in sunflower seeds. I. Lipid peroxidation and membrane damage. Physiol. Plant., 76: 591-597.
Goel, A., Ajay Kumar Goel and Inder Singh Sheoran (2003). Changes in oxidative stress enzymes during artificial ageing in cotton (Gossypium hirsutum L.) seeds, J. Plant Physiol., 160: 1093â€“1100.
Jayaraman, J. (1981). In: Laboratory manual in Biochemistry. Willey Eastern Limited, New Delhi, pp133.
Kakde, R.B. and Chavan, A.M. (2011). Extracellular Lipase Enzyme Production by Seed-borne Fungi Under the Influenc of Physical factors. Intern. J. of Biology, 3(1): 94-100.
Loycrajjou, Y., Lovigny, P.C. Steven, M, Belghazi, C, Job and Job, D. (2008). Proteome wide characterization of seed ageing in arabidopsis. A comparison between artificial and natural ageing. Prot. Pl. Phy., 148: 620â€“41.
Malik, C.P. and Singh, M.B. (1980). Plant enzymology and histo enzymology. Kalyani Publishers, New Delhi.
Mehrotra, N. (2011). Commodity Specific Study in groundnut. Department of Economic Analysis and Research, National Bank for Agriculture and Rural Development, Mumbai. pp 7-8.
Pallavi, M., Sudheer, S.K, Dangi, K.S. and Reddy, A.V. (2003). Effect of seed ageing on physiological, biochemical and yield attributes in sunflower (Helianthus Annus L.) cv. Morden. Seed Res., 31 (2): 161â€“168.
Panse, V.G. and Sukhatme, P.V. (1967). Statistical methods for Agricultural workers. ICAR., Pub., New Delhi.
Povolotskaya, K.L. and Sedenka, M. (1956). A method for collective determination of ascorbic acid, polyphenol and peroxidase activities. Biochemical., 21: 133-136.
Rao, R.G.S., Singh, P.M. and Mathura Rai. (2006). Storability of onion seeds and effects of packaging and storage conditions on viability and vigour. Scientia Horticulture, 110: 1â€“6.
Reddy, S.E.C., Sudhakar, C. and Eswara Reddy, N.P. (2011). Aflatoxin contamination in groundnut induced by aspergillus flavus type fungi: a critical review. International journal of applied biology and pharmaceutical technology, 2(2):180-192.
Scialabba, A., Bellani, L.M. and Dellâ€™aquila, A. (2002). Effects of ageing on peroxidase activity and localization in radish (Raphanus sativus L.) seeds. European Journal of Histochemistry, 46 (4): 351â€“358.
Sung, J.M. and Chiub, C.C. (1995). Lipid peroxidation and peroxide-scavenging enzymes of naturally aged soybean seed. Plant Science, 110: 45-52.
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