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K. K. Sharma U. S. Singh

Abstract

The genus Trichoderma contains species that are of a great economic importance due to their ability to act as biological control agents against a large variety of fungal plant pathogens. In the present investigation thirty isolates of the Trichoderma sp. were obtained from the rhizosphere soils of different plants at different locations at Nainital, Almora, Udham Singh Nagar, Derhadun, Haridwar and Tehri Garhwal districts of Uttarakhand (India). The isolates were characterized on the basis of their cultural and morphological characteristics. The cultural characteristics included linear growth, colony colour, pigmentation and growth pattern. Morphological characteristics studied were structure, shape and arrangement of conidiophores, phialides and conidia. Out of thirty isolates, 6 isolates namely PB10, PB13, PB23, PB26, PB27 and PB28 were identified as T. virens and remaining 24 isolates as T. harzianum.

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Keywords

Characterization, Cultural, Morphological, Phialides, Trichoderma

References
Anees, M., Tronsmo, A., Edel-Hermann, V., Gordon, H.L., Héraud, C. and Steinberg, C. (2010). Characterization of field isolates of Trichoderma antagonistic against Rhizoctonia solani. Fungal Biol., 114:691–701.
Bisset, J. (1991c). A revision of the genus Trichoderma. IV Additional notes on section Longibrachitum. Canadian Journal of Botany. 69: 2418-2420.
Bisset, J. (1991a). A revision of the genus Trichoderma. II. Infrageneric classification. Canadian Journal of Botany. 69: 2357-2372.
Bisset, J. (1991b). A revision of the genus Trichoderma. III. Section Pachybasium. Canadian Journal of Botany. 69: 2373-2417.
Choi, In-Young, Seung-Beom Hong and Yadav, M.C. (2003). Molecular and morphological characterization of green mold, Trichoderma spp. isolated from oyster mushrooms. Mycobiology, 31(2): 74-80.
Elad, Y., Chet, L. and Henis, Y. (1981). A selective medium for improving quantitative isolation of Trichoderma spp. from soil. Phytoparasitica, 9 (1): 59-67.
Gams, W. and Bissett, J. (1998). Morphology and Identification of Trichoderma. In: Trichoderma and Gliocladium, Basic Biology, Taxonomy and Genetics, Harman, G.E. and C.P. Kubicek (Eds.). Vol. 1, Taylor and Francis, London, UK., pp: 3-34.
Gams, W. and Bissett, J. (2002). Morphology and identification of Trichoderma. In: Kubicek, C.P. and Harman, G.E. (eds.). Trichoderma and Gliocladium: Basic biology, taxonomy and genetics. Taylor & Francis Ltd, pp. 3-31.
Krassilnikov, N.A. (1950). Actinomycetes-antagonists and antibiotic substances (in Russian). Academy of Sciences, U.S.S.R., Mascow, Leningrad.
Lin, X. and Heitman, J. (2005). Chlamydospore formation during hyphal growth in Cryptococcus neoformans. Eukaryotic Cell, 4: 1746-1754.
Rahman, A., Begum, M.F., Rahman, M., Bari, M.A., Ilias, G.N.M. and Alam, M.F. (2011). Isolation and identification of Trichoderma species from different habitats and their use for bioconversion of solid waste. Turk. J. Biol., 35:183-194.
Rifai, M.A. (1969). A revision of the genus Trichoderma. Mycological Papers. 116: 1-56.
Samuels, G.J., Petrini, O. and Manguin, S. (1996). Trichoderma: a review of biology and systematic of the genus. Mycol. Res., 100: 923-935.
Samuels, G.J., Chaverri, P., Farr, D.F. and McCray, E.B. (2002a). Trichoderma Online. Systematic Mycology and Microbiology Laboratory, ARS, USDA; Retrieved 24, August 2014 from http://nt.ars-grin.gov/taxadescriptions/keys/TrichodermaIndex.cfm
Samuels, G.J., Dodd, S.L., Gams, W., Castleburry, L.A. and Petrini, O. (2002). Trichoderma species associated with the green mold epidemic of commercially grown Agaricus bisporus. Mycologia, 94: 146-170.
Seaby, D. (1996). Differentiation of Trichoderma taxa associated with mushroom production. Plant Pathol., 45: 905–912.
Shah, S., Nasreen, S. and Sheikh, P.A. (2012). Cultural and morphological characterization of Trichoderma spp. associated with green mold disease of Pleurotus spp. in Kashmir. Research Journal of Microbiology, 7: 139-144.
Singh, S.K., Sharma, V.P., Sharma, S.R., Kumar, S. and Tiwari, M. (2006). Molecular characterization of Trichoderma taxa causing green mould disease in edible mushrooms. Current Science, 90 (3): 427-431.
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Sharma, K. K., & Singh, U. S. (2014). Cultural and morphological characterization of rhizospheric isolates of fungal antagonist Trichoderma. Journal of Applied and Natural Science, 6(2), 451-456. https://doi.org/10.31018/jans.v6i2.481
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