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Z. Aiysha Thasneem K. Aravindh M. Jeba Malar Fencia C. Nitheesh Kumar T. Pavithra K. Rajkumar S. Surendran M. Vidhya R. Mahesh S. Ramalakshmi

Abstract

The rhizosphere of plant possesses important microflora, which secretes wide chemical compounds including secondary metabolites necessary for plant growth and development. The microbial flora of alfalfa plant rhizosphere soil region was explored for functional activity and we found upto ten different pigmented colonies. Due to good functional diversity, this yellow pigmented colony was taken for further studies. Thus, the culture was molecularly characterized and identified for potent bioactive components responsible for antimicrobial activity. The selected culture mass was cultured and secondary metabolites were produced and extracted using ethyl acetate and subjected to GC-MS analysis. The antimicrobial study revealed selective activity against Streptococcus pneumonia, and Proteus sp with zone of inhibition to be 18 and 20 mm respectivelyMolecular identification of the isolate by 16S rRNA sequencing showed the isolate as Macrococcus equipercicus with 100 % similarity. Based on GC-MS analysis report 25bioactive compounds were identified and 13-docosenamide, hexadecanoic acid esters and quercetin were found in ethyl acetate extract. Conclusion: Thus the yellow pigmented gram positive cocci M.equipercicus isolated from Medicago sativa possessed wide antibacterial activity due to presence of quercetin. Through the studies, we were able to identify potent antibacterial compound producing bacteria from M. sativa plant rhizosphere soil.

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Keywords

Alfalfa plant, gas chromatography-mass spectrometry, Macrococcus equipercicus, Medicago sativa, rhizosphere

References
Arora, S., Nandi, D., Prasad, N., Rawat, S. and Pandeya, A, (2013). Isolation and characterisation of antibiotic producing microbes present in rhizospheric soil. International Journal of Scientific & Engineering Research, 4(9):1157-1166.
Becker, K., Heilmann, C., Peters, G. (2014). Coagulase-negative Staphylococci. Clinical Microbiology Revie,ws 27(4): 870 –926.
Bertin, C., Yang, X., and Weston, L. A. (2003). The role of root exudates and allelochemicals in the rhizosphere. Plant and Soil, 256(1): 67-83.
Bora, K. S., Anupam Sharma (2011). Phytochemical and pharmacological potential of Medicago sativa: A review. Pharma Biol.,49(2): 211-220.
Janani, B., Kiruthika, P., Angayarkanni, J. (2014). Isolation Of Pigmented Marine Bacteria Exiguobacterium Sp. From Peninsular Region Of India And A Study On Biological Activity Of Purified Pigment. International Journal of Scientific & Technology Research, 3(3): 375-384
Kalpana Devi, R., Subramani, V., Annamalai, P., Nakulan, Vr., Narayanaperumal, J. (2016). Phytochemical analysis, in vitro antioxidant potential and gas chromatography-mass spectrometry studies of Dicranopterislinearis. Asian J. Pharm. Clin. Res., 9(2): 220-225.DOI: http://dx.doi.org/10.22159/ajpcr.2016.v9s2.13636
Krakowska, A., Ska, K.R., Walczak, J., Kowalkowski, T., Buszewsk, B. (2017). Comparison of Various Extraction Techniques of Medicago sativa: Yield, Antioxidant Activity, and Content of Phytochemical Constituents. J AOAC International, 100(6): 1681-93.doi: 10.5740/jaoacint.17-0234.
Nisha, M. Nair, Kanthasamy, R., Mahesh, R., IruthayaKalaiSelvam, S. and Ramalakshmi, S. (2019a). Identification of Antibacterial Compound from Bacillus horikoshii, isolated from rhizosphere region of Alfalfa plant. Journal of Applied Sciences, 19(2): 140-147.DOI: 10.3923/jas.2019.140.147
Nisha, M. Nair, Kanthasamy, R., Mahesh, R., IruthayaKalaiSelvam, S. and Ramalakshmi, S. (2019b). Production and Characterization of Antimicrobials from isolate PantoeaAgglomerans of Alfalfa Plant Rhizosphere Soil. Journal of Applied and Natural Science, 11(2): 267-272.Doi: https://doi.org/10.31018/jans.v11i2.2031
Sharmila, M., Rajeswari, M., Jayashree, I. (2017). GC-MS Analysis of Bioactive Compounds in the Whole Plant of Ethanolic Extract of Ludwigiaperennis L. Int J Pharm Sci Rev Res 46(1): 124-128.
Zhang, L., Zhang, D., Feng, K. (2006). Inhibition of refined components of Medicago sativa polysaccharides to the activities of reverse transcriptase of HIV and protease of HIV. Zhongguo Shipin Xuebao, 6:5-62
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Aiysha Thasneem, Z., Aravindh, K., Jeba Malar Fencia, M., Nitheesh Kumar, C., Pavithra, T., Rajkumar, K., Surendran, S., Vidhya, M., Mahesh , R., & Ramalakshmi, S. (2019). GC-MS analysis of yellow pigmented Macrococcus equipercicus isolated from alfalfa rhizosphere soil fields of Coimbatore. Journal of Applied and Natural Science, 11(3), 645-649. https://doi.org/10.31018/jans.v11i3.2139
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