The present study was carried out for the evaluation of in-vitro antioxidant potential of alcoholic stem bark (ALSB) extract of Bauhinia variegata Linn. Dried stem bark extract of B. variegata Linn. was screened to evaluate its free radical scavenging effect. Various methods like DPPH assay, reducing power assay, percentage scavenging activity of hydrogen peroxide and superoxide radical scavenging activity were used for screening in-vitro antioxidant potential. Antioxidant potentials were concentration dependent which were compared with standard antioxidants such as butylated hydroxyanisole (BHA) and ascorbic acid. The maximum scavenging effect of B. variegata Linn. alcoholic stem bark extract on DPPH free radical, superoxide radical and hydrogen peroxide was 72.19 + 0.20, 81.60 + 0.22 and 76.06 + 0.16 respectively at a concentration of 2500 ?g/mL. It was clearly indicated that the alcoholic extract of the stem bark has significant in vitro antioxidant activity. Currently available synthetic antioxidants like butylated hydroxyanisole (BHA), butylated hydroxyl toluene (BHT), Ascorbic acid and gallic acid appear to be associated with hepatotoxicity and many others negative health effects. Therefore, natural antioxidants may be preferred over the synthetic antioxidants.
Alcoholic extract, Antioxidant activity, Bauhinia variegata Linn, Scavenging potential, Stem bark
Bansal, V., Malviya, R., Deeksha, Malviya, T. and Sharma, P.K. (2014). Phytochemical, pharmacological profile and commercial utility of tropically distributed plant Bauhinia variegata. Global journal of pharmacology, 8(2) : 196-205.
Bhattacharjee,P., Hullatti, K.K., and Vijay Kumar,M.L. (2011).Athelmintic and antioxidant activity of alcoholic extracts of different parts of Coleus Amboinicus Lour. International Journal of Research in Ayurveda and Pharmacy, 2(1):181-185.
Chidi, N.I. (2014). Heavy metal contamination of herbal medicinal products and cosmetics: A course for concern. Pelagia Research Library, 5(2) : 9-22.
Dhale, D.A. (2011). Phytochemical screening and antimicrobial activity of Bauhinia variegata Linn. Journal of Ecobiotechnology, 3(9) : 04-07.
Johora, S., Kabir, A.S., Hakim, A. , Abdullah ,M. , Islam, N. and Reza, A. (2013). Evaluation of the antioxidant activity of ficus racemosa plant extracts from north-western district of Bangladesh. Journal of Life and Earth science, 8 : 93-99.
Kew, K.S., Neivashini, M., Ooi, X.C., Nabila, P. and Khan, N.H. (2018). Qualitative study on the phytochemical constituents of the flower buds of Bauhinia variegata. Madridge Journal of Pharmaceutical Research, 2(1) : 47-51.
Kumari,R., Kumar, S., Kumar, A., Kumar, K. and Dubey, R.C.(2017). Antibacterial, antioxidant and Immunomodulatory properties in extracts of Barleria lupulina Lindl. BMC Complementary and Alternative Medicine, 17(484) : 1-11.
Labiad, M.H., Harhar, H.,Ghanimi, A., and Tabyaoui, M. (2017). Phytochemical screening and antioxidant activity of Moroccan thymus satureioides extracts. Journal of Materials and Environmental Sciences, 8(6) : 2132-2139.
Najat,A. B., Reem, A. A., and Mohammed M. I.(2015). Phytochemical and taxonomic evaluation of Rhazya stricta in Saudi Arabia. Saudi journal of biological sciences,24(7): 1513-1521
Naskar,S., Islam,A., Mazumder,U. K., Saha,P., Haldar,P. K., and Gupta, M.(2010). In Vitro and In Vivo Antioxidant Potential of Hydromethanolic Extract of Phoenix dactylifera Fruits. Journal of Scientific Research, 2(1) :144-157.
Nuraniye,E., Mehmet, A., Ozge, Ç., and Mehmet, T. (2017). Investigating of Phytochemicals, Antioxidant, Antimicrobial and Proliferative Properties of Different Extracts of Thymus spathulifolius Hausskn. and Velen. Endemic Medicinal Plant from Sivas, Turkey . International Journal of Secondary Metabolite, 4(3) : 155-166.
Rubaiyat Hasan, M.d., Nasirul Islam, M.d. and Rokibul Islam, M.d. (2016). Phytochemistry, pharmacological activities and traditional uses of Emblica officinalis: A review . International Current Pharmaceutical Journal, 5(2), 14-21.
Sahu, G. and Gupta, P.K. (2012). A review on Bauhinia Variegata Linn. International Research Journal of Pharmacy, 3(1): 48-51.
Trease, G.E. and Evans, W.C. (1983). Textbook of pharmacognosy. 12th Edition, Tindall and Co., London.
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
This work is licensed under Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) © Author (s)