In the present study, wild-type Drosophila melanogaster collected from stock culture were sub-cultured in three different types of solid culture media (corn, barley and wheat) and control medium for two weeks to produce F1 generation. The duration of larval and pupal development, number of pupal cases and hatched flies were scored for first generation. The results were analyzed by using one-way ANOVA, Bonferroni multiple comparison test and paired sample t-test. The control medium showed no pupal cases and hatched flies. Among all the three solid culture media tested, corn meal, barley meal and wheat meal, the latter showed highly significant results at p?0.001 than others. However, this parameter was not affected by the carbohydrate amount in the media. The present investigation is an attempt to evaluate the influence of different formulated solid culture media on the life span and reproduction of fruit flies.
ANOVA, Drosophila melanogaster, Fecundity, Pupa, Solid culture media
Ashburner, M. and Roote, J. (2000). Laboratory culture of Drosophila. In: Drosophila protocols, ed. W. Sullivan, M. Ashburner, and R. S. Hawley, Cold Spring Harbor Laboratory Press, Cold Spring Harbor,NY, pp 585-599.
Baumberger, J. (1917). Solid media for rearing Drosophila. The American Naturalist, 51: 447-448.
Bridges, C. and Darby, H. (1993). Culture media for Drosophila and the pH of media. TheAmerican Naturalist, 67: 437-472.
Burnell, A. N., Reaper, C. and Doherty, J. (1991). The Effect of Acclimation Temperature on Enzyme Activity in Drosophila melanogaster. Comparative Biochemistry and Physiology Part B: Comparative Biochemistry, 98(4): 609-614.
Catchpoole, D. 2005. Too dry for a fly. Creation, 28(1):34-35.
Dermerec, M. (1950). Biology of Drosophila. New York: John Wiley and Sons, Inc,pp 632.
Demerec, M. And Kaufman, B.P. (1996). Drosophila guide: introductiont to the genetics and Cytology of Drosophila melanogaster.10thed. CarnegieInstituteofWashington,USA, pp 1-27.
Dirkson, R. (2009). A Drosophila Protocol for Teaching Homeobox Genetics and Homeotics Mutations. http://www.slideshare.net/RDirksen/drosophila-protocol.
Fay, H.A.C. (1989). Multi-host species of fruit fly. In: World Crop Pest. Fruit flies, their biology, natural enemies and control. Edited by Robinson A.S. and Hooper G. Ed. Elsevier, New York,3b:129-138.
Flagg, R. O. (1998). CarolinaTM Drosophila Manual. Carolina Biological Supply Company, Burlington, NC, pp 1-23.
Geiger, P. (2002). An Introduction to Drosophila melanogaster. http://biology.arizona.edu/sciconn/lessons2/geiger/intro.html.
Gilbert, S. F. (2000). Developmental Biology.6th ed. Sunderland: Sinauer Associates, pp 294-296.
Griffiths, A. J., Miller, J. H., Suzuki, D. T., Lewontin, R. C. and Gelbart, W. M. (2000). sex chromosomes and sex-linked inheritance. An Introduction to Genetic Analysis 7th ed. W. H. Freeman. New York.
Henschen, F. (1933). The Nobel Prize in Physiology or Medicine,1933 Thomas H. Morgan-AwardCeremonySpeech.https://www.nobelprize.org/nobel_prizes/medicine/laureates/1933/.
Jaenike, J. (1986). Feeding behavior of future fecundity in Drosophila. The American Naturalist, 127:118-123
Keller, A . 2007. Drosophila melanogaster’s history as a human commensal. Curr Biol17: 77 -81.
Kenney, D. E. and Borisy, G. G. 2009. Thomas Hunt Morgan at the Marine Biological Laboratory: Naturalist and Experimentalist. Genetics, 181(3):841-846.
Lushchak, O.V., Gospodaryov, D.V ., Rovenko, B.M . (2012). Balance between macronutrients affects life span and functional senescence in fruit fly Drosophila melanogaster. J Gerontol, 67:118-125.
Lushchak, O.V., Gospodaryov, D.V., Rovenko, B.M., Yurkeyych, J.S., Perkhulyn, N.V. and Lushchak, V.I., (2014). Specific dietary carbohydrates differentially influence the life span and fecundity of Drosophila melanogaster. J Gerontol., 69 (1): 3-12.
Markow, T. A. and O’Grady, P. M. 2006. Distribution in Drosophila. A Guide to Species Identification and Use. California, Elsevier.
Mitrovski, P. and Hoffman, A. A. 2001. Postponed Reproduction as an Adaptation to Winter Conditions in Drosophila melanogaster: Evidence for Clinical Variation under Semi-Natural Conditions. Proceedings: Biological Sciences, 268(1481):2163-2168.
Parvathi, D., Amritha, A. and Paul, S.F.D. 2009. Wonder animal model for genetics-Drosophila melanogaster Its life cycle and breeding methods- A review. Sri Ramachandra Journal of Medicine, 2(2):33-38
Purves, W. K., Orians, G. H., Heller, H. C. and Sadava, D. 1998. Life: The Science of Biology. Sinauer Associates. Sunderland.
Ranganath, H. A. (1999). Teaching and learning genetics with Drosophila. http://www.ias.ac.in/resonance/Feb1999/pdf/Feb1999Classroom.pdf.
Remize, F., Roustan, J. L., Sablayrolles, J. M., Barre, P. and Dequin, S. (1998). Glycerol Overproduction by Engineered Saccharomyces cerevisiae Wine Yeast Strains Leads to Substantial Changes in By-Product Formation and to a Stimulation of Fermentation Rate in Stationary Phase. Applied and Environmental Microbiology, 65(1): 143-149.
Robertson, F., and Sang, J. (1944). Theecological determinants of population growth ina Drosophila culture. I. Fecundity of adult fruitflies. Proceedings of the Royal Society of London. Series B, Biological Sciences, 132:258-277.
Sandhyarani, N. (2010). Drosophila melanogaster Life Cycle. http://www.buzzle.com/articles/drosophila-melanogaster-life-cycle.html.
Sang, J. H. (1956). The Quantitative Nutritional Requirements of Drosophila melanogaster. Journal of Experimental Biology, 33:45-72.
Skirvin, R. M., Chu, M. C., Mann, M. L., Young, H., Sullivan, J. and Fermanian, T. (1986). Stability of tissue culture medium pH as a function of autoclaving, time, and cultured plant material. Plant Cell Reports, 5: 292-294.
Smith, M. L. (1932). The effect of heat on sugar solutions used for culture media. Biochem J., 26(5):1467-1472.
Spencer, W. P. (1943). Drosophila culture with a minimum of agar. The Ohio Journal of Science, 43 (4):174-175.
Stocker, H. and Gallant, P. (2007). Getting Started: An Overview on Raising and Using Drosophila. In Drosophila: Methods and Protocols. New York: Humana Press Inc.
Tatum, E.(1939). Nutritional requirements ofDrosophila melanogaster. Proceedings of the
National Academy of Science of the United States of America, 25: 490-497.
Tee Sui Yee. (2010). Optimization of fruit fly (Drosophila melanogaster) Culture media for higher yield of offspring. Universiti Tunku Abdul Rahman, pp 1-57.
Uysal, H., Aydogan, M. N. and Algur, O. F. 2002. Effect of single cell protein as a protein source in Drosophila Culture. Brazilian Journal of Microbiology, 33: 314-317.
Wayne, M. L., Soundararajan, U. and Harshman, L. G. (2006). Environmental stress and reproduction in Drosophila melanogaster: starvation resistance, ovariole numbers and early age egg production. EMC Evolutionary Biology,6:57.
Widdowson, E. M.and McCance, R.A. (1935).The available carbohydrate of fruits:determination of glucose, fructose, sucrose and starch, Biochem J., 129 :151-156.
Wollard, L., Klein B., Carlson, J.D. and Carlson, K.A. (2006). Rearing media as a variable in fruit fly fecundity: An activity to introduce scientific methods of inquiry to biology students. Bioscene, 32(3):24-29.
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
This work is licensed under Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) © Author (s)