##plugins.themes.bootstrap3.article.main##

Erayya SL Nandani Shukla Kahkashan Arzoo J. Kumar

Abstract

In vitro efficacy of twenty five Trichoderma isolates (twenty were TCMS series viz., TCMS 2, 4, 5, 12, 14a, 14b, 15, 16, 24, 32, 34, 36, 43, 60, 62, 64, 65, 72, 85 and 93, and five Th series; Th 1, 3, 14, 19 and 32) were ascertained for their antagonistic activity against few major plant pathogenic oomycetes namely, Phytophthora infestans, P. parasitica and Pythium aphenidermatum using dual culture technique. P. infestans was isolated from infected potato leaves and Pythium aphenidermatum from infected brinjal. P. parasitica culture was collected from Central Potato Research Institute (CPRI), Simla. The present study was conducted at Biological Control Laboratory, Department of Plant Pathology, G.B. Pant University of Agriculture and Technology, Pantnagar. All the 25 Trichodrma isolates were found significantly effective against the test pathogens. TCMS-36 and TCMS-72 were found highly effective against P. aphinidermatum with 59.57 per cent inhibition of radial growth of the fungus. Maximum reduction in mycelial growth of P. infestans was recorded with isolate TCMS-64 (60.40%) followed by TCMS-65 (59.41%), TCMS-34 (58.42%), TCMS-24, TCMS-43 and TCMS-93 with 57.43 per cent inhibition. While, maximum inhibition of P. parasitica was recorded with TCMS-4 (92.75%) followed by TCMS-36 (92.23%), TCMS-2 (91.71%), TCMS-14a (91.17%) and TCMS-32 (90.67%). The selected potential isolates may be applied to sustainable and eco-friendly management of many major crop diseases caused by the oomycetes and other fungi.

Downloads

Download data is not yet available.

##plugins.themes.bootstrap3.article.details##

##plugins.themes.bootstrap3.article.details##

Keywords

Antagonism, Bioagent, Dual culture, Management, Oomycetes

References
Benitez, T., Limon, J., Delgado Jarana, J. and Rey, M. (1998). Glucanolytic and other enzymes and their control. In: Trichoderma and Gliocladium, Vol. 2. G.E. Harman and C.P. Kubicek, eds. Taylor and Francis, London. pp. 101-127.
Benitez, T., Rincón, A.M., Limón, M.C. and Codón A.C. (2004). Biocontrol mechanisms of Trichoderma strains, Int. Microbiol. 7: 249-260.
Brunner, K., Peterbauer, C.K., Mach, R.L., Lorito, M., Zeilinger, S. and Kubicek, C.P. (2003). The Nag1 N-acetylglucosaminidase of Trichoderma atroviride is essential for chitnase induction by chitin and of major relevance to biocontrol. Curr.Genet. 43: 289-295.
Chaube, H.S., Mishra, D.S., Varshney, S. and Singh, U.S. (2002). Biological control of plant pathogens by fungal antagonists: Historical background, present status and future prospects. Annu. Rev. Plant Pathol. 2: 1-42.
Chet, I., Harman, G.E. and Baker, R. (1981). Trichoderma harzianum. its hyphal interactions with Rhizoctonia solani and Pythium spp. Microb. Ecol. 7: 29-38.
Cook, R.J. and Baker, K.F. (1983). The Nature and Practice of Biological Control of Plant Pathogens. The American Phyto-pathological Society. St. Paul. Minnesota. pp. 539.
El-Kafrawy, A. A. (2002). Biological control of bean damping-off caused by Rhizoctonia solani. Egyptian Journal Agricultural Research 80 (1): 57-70.
Freeman, S., Maymon, M., Kirshner, B., Rav, D. and Elad, Y. (2002). Use of GUS transformants of Trichoderma harzianum isolate T39 (TRICHODEX) for studying interactions on leaf surfaces. Biocontrol Sci. Tech. 12 (3): 401-407.
Howell, C.R. (2002). Cotton seedling pre-emergence damping-off incited by Rhizopus oryzae and Pythium spp. and its biological control with Trichoderma spp. Phytopathology 92: 177-180.
Inbar, J., Menendez, A. and Chet, I. (1996). Hyphal interaction between Trichoderma harzianum and Sclerotinia sclerotiorum and its role in biological control. Soil Bio.Biochem. 28 (6): 757-763.
Joshi, B.B., Bhatt, R.P. and Bahukhandi, D. (2010). Antagonistic and plant growth activity of Trichoderma isolates of Western Himalayas Journal of Environmental Biology 31(6): 921-928.
Matroudi, S., Zamani, M.R and Motallebi, M. (2009). Antagonistic effects of three species of Trichoderma sp. on Sclerotinia sclerotiorum, the causal agent of canola stem rot. Egyptian Journal of Biology, 11:37-44
Mendoza-Mendoza, A., Pozo, M.J., Grzegorski, D., Martinez, P., Garcia, J.M., Olmedo-Monfil, V., Cortés, C., Kenerley, C., and Herrera-Estrella, A. (2003). Enhanced biocontrol activity of Trichoderma through inactivation of a mitogen-activated protein kinase. Proceedings of the National Acacemy of Sciences of the United States of America 100: 15965-15970.
Mishra, B.K., Rohit, Kumar Mishra.. Mishra, R.C. Amit Kumar, Tiwari, Ramesh Singh, Yadav and Anupam Dikshit. (2011). Biocontrol efficacy of Trichoderma viride isolates against fungal plant pathogens causing disease in Vigna radiata L. Archives of Applied Science Research 3 (2): 361-369.
Mukhopadhyay, A.N. and Mukjerjee, P.K. (1998). Biological control of plant diseases: Status in India. In: Biological suppression of phytopathogens, Nematodes and Weeds (Singh, S. P. and Husain, S. S., eds.). Project Directorate of Biological Control, Bangalore.
Muslim, A. Horinouchi, H. and Hyakumachi, M. (2003). Biological control of Fusarium wilt of tomato with hypovirulent binucleate Rhizoctonia in greenhouse conditions. Mycosci. 44:77-84.
Muthu Kumar, A. and Pratibha Sharma. (2011). Molecular and morphological characters: Anappurtenance for antagonism in Trichoderma spp. African Journal of Biotechnology 10(22): 4532-4543.
Poornima Sharma (2011). Complexity of Trichoderma-Fusarium interaction and manifestation of biological control. AJCS. 5(8): 1027-1038.
Rey, M., Delgado-Jarana, J. and Benitez, T. (2001). Improved antifungal activity of a mutant of Trichoderma harzianum CECT 2413 which produces more extracellular proteins. Appl. Microbio.Biotech. 55 (5): 604-608.
Singh, U.S., Mishra, D.S., Prasad, R.D., and Chaube, H.S. (2001a). Biological Control of Plant Pathogens in India: Historical perspectives, present status and future prospects. In: Hundred Years of Biological in India (ed., S. P. Singh), ICAR, New Delhi
Svetlana, Z., Stojanović1, S., Ivanović, Ž., Gavrilović, V., Tatjana, P. and jelica, B. (2010). Screening of antagonistic activity of microorganisms against Colletotrichum acutatum and Colletotrichum gloeosporioides.Arch. Biol. Sci., Belgrade 62 (3): 611-623.
Viterbo, A., Ramot, O., Chernin, L. and Chet, I. (2002). Significance of lytic enzymes from Trichoderma spp. in the biocontrol of fungal plant pathogens. Anton. Leeuw. 81:549-556.
Weindling, R. (1932). Trichoderma lignorum as a parasite of other soil fungi. Phytopathology 22: 834-845.
Citation Format
How to Cite
SL, E., Shukla, N., Arzoo, K., & Kumar, J. (2018). Mass screening of Trichoderma spp. for their antagonism against some plant pathogenic oomycetes fungi. Journal of Applied and Natural Science, 10(3), 813-817. https://doi.org/10.31018/jans.v10i3.1718
More Citation Formats:
Section
Research Articles