Qazi Nyrah Shakil Ahmed Wani Nazima Nazir Mir Nadeem Hassan Shaheen Farooq Zahid Kashooand Shoiab Kamil


he present study has determined prevalence, serological diversity, virulence gene profileand in vitro antibiogram of Escherichia coli (E. coli) isolates from respiratory tract of broiler chicken in Kashmir valley along with molecular cloning of ibeA gene, an important zoonotic among invasion-associated genes responsible for neonatal meningitis in humans. A total of 224 broilerchickens with a history of respiratory tract infections, in a flock size of 2692 birds from organised and unorganised poultry farms of Kashmir valley, were screened for E. coli isolation. The prevalence of E. coli in birds with respiratory tract infection was found to be 6.38 per cent. Serogrouping revealed that the E. coli isolates were typeable into 10 serogroups with prevalence rate of 8.8% for serogroup O2, along with few isolates rough or untypeable. The screening of E. coli isolates for virulence gene profile revealed the presence of iss in (89.53%), iucDin (85.46 %), tsh in (51.74 %), cva/cvi in (33.14 %), irp2in (55.23 %), papCin (33.72 %), vat in (31.39 %), astAin (38.95 %), fimCin (93.60 %) and ibeAin (1.16 %) isolates. The antibiogram screening of E. coli isolates revealed majority of isolates to be resistant to tetracycline (94.71%), ampicillin (92.67%) and cephalaxin (79.67%).However, most of the isolates were sensitive to amikacin (74.41%) followed by amoxacillin-sulbactam (68.60%) and gentamicin (67.44%). The study thus, suggests that the E. coli isolates from respiratory tract infection of poultry harbour differential virulence genes along with the genes of zoonotic interest and variable antimicrobial resistance, thus, opening the areas for devising dynamic, emergent and effective treatment line.


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Antibiogram, Escherichia coli, Prevalence, Virulence genes

Altekruse, S.F., Elvinger, F., DebRoy, C., Pierson, F.W., Eifert, J.D. and Sriranganathan, N. (2002). Pathogenic and Faecal Escherichia coli Strains from Turkeys in a Commercial Operation. Avian Dis,46(3) : 562-569.
Ammar,A.M., Abd El-Aziz, N.K., Nasef, S.A., Bakry, N.R., El Atfehy, N.M.,Erfan, A.M. and Shalaby, A.G. (2014) Use of Multiplex PCR for Detection of Bacterial Respiratory Infections in Poultry. Zag. Vet. J.42 (3) :133-144
Bauer, A.W., Kirby, W.M., Sherris, J.C. and Turck, M. (1966). Antibiotic susceptibility testing by a standardized single disc method. Am J Clin Pathol., 45 : 493-496.
Buchanan, R.E. and Gibbon, N.E. (1994). Burgey’s manual of Determinative Bacteriology. 9th Edition. Williams and Wilkins, Baltimore, pp. 787.
Circella, E., Pennelli, D., Tagliabue, S., Ceruti, R., Giovanardi, D. and Camarda, A. (2009). Virulence-associated genes in Avian Pathogenic Escherichia coli of turkey. Ital. J. Anim. Sci,8 : 775-779.
Dave, C.J., Parmer, H.C., Patel, A.K. and Prajapati, S.K. (2004). Broiler mortality pattern in Gujarat state. XXI Annual Conference of IAVP, p. 42..
Dozois, C.M., Fairbrother, J.M., Harel, J. and Bosse, M. (1992). Pap-and pil-related DNA sequences and other virulence determinants associated with Escherichia coli isolated from septicemic chickens and turkeys. Infect. Immun,60 : 2684-2656.
Ewers, C., Janssen, T., Kiessling, S., Philipp, H. C. and Wieler, L. H. (2004). Molecular Epidemiology of avian pathogenic Escherichia coli (APEC) isolated from colisepticemia in poultry. Vet Microbiol ,104 : 91-101.
Franck, S.M., Bosworth, B.T. and Moon, H.W. (1998).
Multiplex PCR for enterotoxogenic, attaching and
effacing and Shiga toxin-producing Escherichia coli strains from calves. J. Clin. Microbiol,36 : 1795-1797.
Germon, P., Chen, Y.H., He, L., Blanco, J. E., Bree, A., Schouler, C., Huang, S.H. and Moulin-Schouleur, M. (2005). ibeA, a virulence factor of avian pathogenic Escherichia coli. Microbiology,151 : 1179-1186.
Janben, T., Schwarz, C., Preikschat, P., Voss, M., Philipp, H. and Wieler, L. H. (2001). Virulence-associated genes in avian pathogenic E. coli (APEC) isolated from internal organs of poultry having died from colibacillosis. Int. J. Med. Microbiol., 291 : 371-378.
Kabir, S.M.L.(2010) Avian Colibacillosis and Salmonellosis: A Closer Look at Epidemiology, Pathogenesis, Diagnosis, Control and Public Health Concerns. Int. J. Environ. Res. Public Health 7(1): 89-114.
Kim, T.E., Jeong, Y.W., Cho, S.H., Kim, S.J. and Kwon, H.J. (2007). Chronological study of antibiotic
resistances and their relevant genes in Korean avian pathogenic Escherichia coli isolates. J. Clin. Microbiol.,45(10) : 3309-3315.
Omer, M.M., Abusalab, S.M., Gumaa, M.M., Mulla, S.A., Omer, E.A., Jeddah, I.E., Al-Hassan, A.M., Hussein, M.A. and Ahmed, A.M. (2010). Outbreak of colibacillosis among broiler and layer flocks in intensive and semi intensive poultry farms in Kassala state, Eastern Sudan. Asian Journal of Poultry Science,4(4) : 173-181.
Rahman,M.A., Samad, M.A., Rahman,M.B. and Kabir, S.M.L. (2004). Bacterio-pathological Studies on
Salmonellosis, Colibacillosis and Pasteurellosis in Natural and Experimental Infections in Chickens. Bangl. J. Vet. Med. 2(1) : 01-08
Raji, M., Adekeye, J., Kwaga, J., Bale, J. and Henton, M. (2007). Serovars and biochemical characterization of Escherichia coli isolated from colibacillosis cases and dead-in-shell embryos in poultry in Zaria-Nigeria. Vet Arhiv,77(6) : 495-505.
Rodriguez-Siek, K.E., Gidding, C.W., Doetkott, C., Johnson, T.J. and Nolan, L.K. (2005). Characterizing the APEC pathotypes. Vet Res,36 :241-256.
Saidi, B., Mafirakureva, P. and Mbanga, J. (2013). Antimicrobial resistance of Escherichia coli isolated from chickens with colibacillosis in and around Harare,
Zimbabwe. Avian Dis,57(1) : 152-154.
Salehi, T.Z. and Bonab, S.F. (2006). Antibiotics susceptibility pattern of Escherichia coli strains isolated from
chickens with colisepticemia in Tabriz Province, Iran. Int. J. Poult. Sci.,5(7) : 677-684.
Sambrook, J., Russel, D.W. (2001). Molecular Cloning- A Laboratory Manual. Cold Spring Harbour,New York, USA.
Sarpe, B., Biradar, B.P., Kulkarni, G.B., Bhise, D.W., Kondre, B.M., Rathode, P.R. and Dhaygude, V.S. (2009).
Mortality pattern in broilers. Proceedings XXVI Annual Conference of IAVP. AD-36, p. 150.
Sharada, R., Ruban, S.W. and Thiyageeswaran, M. (2010). Isolation, characterization and antibiotic resistance
pattern of Escherichia coli isolated from poultry.
American-Eurasian Journal of Scientific Research 5(1) : 18-22.
Schouler, C., Schaeffer, B., Brée, A., Mora, A., Dahbi, G., Biet, F., Oswald, E., Mainil, J., Blanco, J. and
Moulin-Schouleura, M. (2012). Diagnostic Strategy for Identifying Avian Pathogenic Escherichia coli Based on Four Patterns of Virulence Genes. Journal of Clinical Microbiology, 50(5): 1673-1678
Wang, X.M., Liao, X.P., Zhang, W. J., Jiang, H.X., Sun, J., Zhang, M.J., He, X.F., Lao, D.X. and Liu, Y.H. (2010). Prevalence of serogroups, virulence genotypes,
antimicrobial resistance, and phylogenetic background of avian pathogenic Escherichia coli in south of China. Foodborne Pathog Dis., 7(9) : 1099-1106.
Yaguchi, K., Ogitani, T., Osawa, R., Kawano, M., Kokumai, N., Kaneshige, T., Noro, T., Masubuchi, K. and
Shimizu, Y. (2007). Virulence factors of avian
pathogenic Escherichia coli strains isolated from chickens with colisepticemia in Japan. Avian Dis, 51 (3) : 656-662.
Yamamoto, T. and Echeverria, P. (1996). Detection of the enteroaggregative Escherichia coli heat-stable
enterotoxin 1 gene sequences in enterotoxigenic E. coli strains pathogenic for humans. Infect. Immun,64 :1441-1445.
Yang, H., Chen, S.., White, D.G., Zhao, S., McDermott, P., Walker, R. and Meng, J. (2004). Characterization of multiple-antimicrobial-resistant Escherichia coli
isolates from diseased chickens and swine in China. J. Clin. Microbiol.42: 3483-3489.
Zhao, S., Maurer, J.J., Hubert, S., De Villena, J.F., McDermott, P.F., Meng, J., Ayers, S., English, L. and White, D.G. 2005. Antimicrobial susceptibility and molecular
characterization of avian pathogenic Escherichia coli isolates. Vet Microbiol, 107 : 215-224.
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Nyrah, Q., Wani, S. A., Nazir, N., Hassan, M. N., Farooq, S., & Kamil, Z. K. S. (2017). Isolation, virulence gene profiling with molecular cloning of ibeA gene and antibiogram of Escherichia coli from respiratory tract infections of broiler chicken in Kashmir, India. Journal of Applied and Natural Science, 9(3), 1710-1717. https://doi.org/10.31018/jans.v9i3.1426
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