Navneet Kaur Dhaliwal Jaspal Singh Pardeep K. Chhuneja


Comparative evaluation of Doolittle, Karl Jenter and Cupkit techniques of Apis mellifera Linnaeus queen bee rearing was done during spring (mid February- mid April 2013) breeding season. The highest acceptance of cell cups (66.00 %), queen cells raising (64.00 %), their sealing (60.67 %) and emergence of gynes (54.67 %) was recorded in Cupkit apparatus. Maximum weight of newly emerged gyne was recorded in Doolittle method in plastic cell cups (212.36 mg), while the mean weight was 184.96 mg in case of Cupkit apparatus. Overall, Cupkit proved to be the best option for queen bee rearing because of its better performance in terms of acceptance of larvae (66.00 %) and the number of successfully produced gynes i.e. 16 queens/colony/cycle of 12 days.


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Apis mellifera, Cupkit, Doolittle grafting method, Karl Jenter, Queen cell cups

Ahmad S. B. and Dar S. A. (2013). Mass rearing of queen bees, Apis mellifera L. (Hym:Apidae) for bee colony development raised under the temperate conditions of Kashmir. The Bioscan., 8(3): 945-948.
Büchler, R., Andonov, S., Bienefeld, K., Costa, C., Hatjina, F., Kezic, N., Kryger, P., Spivak ,M., Uzunov2 A. and Wilde J. (2013). Standard methods for rearing and selection of Apis mellifera queens. J. Apic. Res., 52(1): 1-30.
Chang, S.Y. (1977). Effects of,size and type of queen cup on the production of royal jelly and acceptance by nurse bees. Master Thesis, National Chung Hsing University Taichung, Taiwan. Apic. Abstr., 2: 201/79.
Dodologlu, A., Emsen, B. and Genc, F. (2004). Comparison of some characteristics of queen honey bees (Apis mellifera L.) reared by usingDoolittle method and natural queen cells. J. App. Anim. Res., 26: 113-115.
El-Din, H.A.S. and Samni, M.A. (1990). Comparison of two types of queen cell cups for commercial royal jelly production in honey bee (Apis mellifera L.) colonies. Honey Bee. Sci., 11: 159-160.
Emsen, B., Dodologlu, A. and Genc, F. (2003). Effect of larvae age andgrafting on the larvae accepted rate and height of sealed queen cell(Apis mellifera L.). J. App. Anim. Res., 24: 201-206.
Gatoria, G.S., Aulakh, R.K., Chhuneja, P.K., Singh, B. and Kamaldeep (2004). Evaluation of queen
cup-kit apparatus for mass rearing of Apis
mellifera Linn. queen bees. Ins. Env., 10(3): 101-103.
Genc, F., Emsen, B. and Dodologlu, A. (2005). Effects of rearing periodand grafting method on the queen bee rearing. J. App. Anim. Res., 27(1): 45-48.
Goodman, R. (2008). Raising Queen Honey Bees.
Agriculture Notes, ID: AG1194. State of Victoria, Department of Primary Industries. ISSN 1329-8062.
McKinley, W.M. (1963). Grafting- how it is done. Glean Bee Cult., 91: 404-409.
Nageh, S. M. O., Mabrouk, M. S. O. and Mohanna, K. M. (2010). Some factors affecting successful Apis mellifera queen rearing in Sohag. J. Agric. Sci., 18(1): 213-19.
Ruttner, F. (1988). Breeding techniques and selection for breeding of the honeybee. British Islets Bee Breeders Association. Derby, UK, pp 152.
Wilde, J., Woyke, J. and Wilde, J. (2002). Rearing honey bee queens in wax and plastic queen cell cups. In:Proceedings of the 6th Asian Apicultural Association International Conference and World Apiexpo,Banglore on 24th Feb- 1st March, 2002,pp, 167.
Skowronek, W., Bieñkowska, M. and Kruk C. (2004). Changes in body weight of honeybee queens
during their maturation. J. Apic. Sci., 48 (2): 61-68.
Woodward, D. (2007). Queen bee: Biology, rearing and breeding. Balclutha; New Zealand. 137 pp.
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Dhaliwal, N. K., Singh, J., & Chhuneja, P. K. (2017). Comparative evaluation of Doolittle, Cupkit and Karl Jenter techniques for rearing Apis mellifera Linnaeus queen bees during breeding season. Journal of Applied and Natural Science, 9(3), 1658-1661. https://doi.org/10.31018/jans.v9i3.1417
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